LABORATORY AUTOMATION SPECIALISTS  
 

 

Beeline SX Accuracy & Precision

What affects Accuracy and Precision?

Performance obtained from liquid handlers depends on many factors including:

Sample related

  Liquid viscosity
  Temperature of sample and local environment
  Sample quality - homogeneity, clots and other particulates

Instrument related

  Syringe size and accuracy of manufacture
  Accuracy and reproducibility of syringe drive
  Needle size and surface area
  Needle coating type, its integrity ans surface energy
  Probe type (Disposable tip or Fixed)
  Syringe speed profiles on aspiration and dispense
  Arm speed profiles
  Tip volume, tip profile, hydrophobicity, surface characteristics
  Pipetting mode (reverse type or blow out)
  Probe positioning during dispense step eg Tip touch onto tube wall, Dispense into existing fluid or non-contact
  System fluid type and % of disolved air, % of detergent such as Tween
  Maintenance status

As can be seen, there are large number of variables to take into consideration. Whilst some can be controlled others cannot. We try to make sure you are aware of the effect of these and provide default profiles and configurations to minimise any optimisation work that may be required.

Some typical performance figures are provided below for a small selection of probe configurations. Owing to the number of parameters affecting performance these are only relevant to the conditions applied during the test but at least provide an indication of what is achievable..

 

Fixed probe: (# 9929/201)

Conditions for the test:
Probe type: Fixed Probe, fluid filled
Syringe Volume: 1000ul
System Fluid: Distilled Water
Sample fluid: Distilled Water
Speed Profiles: Default
Dispense Mode: Contact


Required Volume Inaccuracy (%) Imprecision Limit (% CV)
10 < 7 < 10
25 < 5 < 5
50 < 2.5 < 2.5
100 < 2.0 <2.0

The above figures are obtained during routine testing of Beeline SX instruments in a production environment. The limits applied may differ depending on the application and volumes that the instruments are used for. Note that typical values are always significantly better than those stated as limits.

 

Disposable Tip probe: (Part number 9929/201)

Conditions for the test:
Probe type: Disposable Tip (#9967/201)
Adaptor Type: Multi-Tip Adaptor (#6305/033)
Syringe Volume: 100ul
System Fluid: Distilled Water
Sample fluid: Distilled Water
Speed Profiles: Default
Dispense Mode: Contact
Disposable Tip: #TIP1007

Tip Size: 15ul

Required Volume Inaccuracy (%) Imprecision Limit (% CV)
1 <10.0 < 5.0
5 < 5.0 <2.0
10 < 2.5 <1.0

 

Disposable Tip probe: (Part number 9929/201)

Conditions for the test:
Probe type: Disposable Tip (#9967/201)
Adaptor Type: Multi-Tip Adaptor (#6305/033)
Syringe Volume: 500 ul
System Fluid: Distilled Water
Sample fluid: Distilled Water
Speed Profiles: Default
Dispense Mode: Contact
Disposable Tip: #TIP1009

Tip Size: 125ul

Required Volume Inaccuracy (%) Imprecision Limit (% CV)
5 <5.0 <3.0
25 <2.0 <1.5
100 <1.5 <1.0

 

 

 

 

 

 

 

 

 


IFA Automation Blood Grouping Sample Processors Diluters Support OEM
Site Home Directions Key Contacts      

Copyright © 2003-2021   HTZ Ltd.

Site last updated: January 28th 2021

HTZ Limited:

Registration Number: 05746732
R
egistered Office: Unit 4, The Felbridge Centre, Imberhorne Lane, East Grinstead, West Sussex, RH19 1XP